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The world’s first mass producer and A Global supplier of “Trehalose”

Also called mushroom sugar

Trehalose – unique, low-yield carbohydrate

Trehalose is a naturally occurring carbohydrate that can be produced by a number of microscopic animals and plants. It is particularly abundant in mushrooms, therefore, it has historically been called mushroom sugar.
Trehalose is usually produced by these animals and plants in higher concentrations in response to environmental stress conditions such as dehydration or freezing. As drying or freezing occurs water is drawn away from important biologic structures in the cells. If this happens to a high degree it can be fatal to the cells and ultimately to the organism. The trehalose can replace the water during these times and spare the organism from irreversible damage.
Owing to its intriguing characteristics, trehalose was expected to have numerous uses, however, its production because it was manufactured by extraction from yeast, which results in relatively low yields. Other methods of production, such as a fermentative using microorganisms, and genetic modification of plants by inserting enzyme genes were developed, but none of them resulted in commercially viable production yields.
As a result, trehalose was sold at a price around 30,000 to 50,000 yen/kg. This cost could be justified in some pharmaceuticals or cosmetics, but could only rarely be used for processing food. Creating a manufacturing process that would lower the price of trehalose was a highly sought technology.

Part of the soil samples collected

Kazuhiko Maruta discovered a microorganism, which generates a trehalose-producing enzymes.

Over the years, Hayashibara Co., Ltd., a research and development company specializing in the production of syrups and sugars, has conducted a variety of food science research using enzymes from microorganisms.

The company has a long tradition of giving a small bag and a spoon to all of its employees and encouraging them to bring back soil samples from their travels far and wide. This micro-treasure hunt is a way to broaden the reach of the company’s researchers, the hope being that the soil brought home might contain as yet undiscovered microorganisms and enzymes that will prove useful in the company’s syrup and sugar production.

In early 1990’s, one night, one of the young researchers in the company, Kazuhiko Maruta, dreamt of a “sparkling colony” (a visible mass of a single species of microorganisms). Two days later, a microorganism that produces trehalose producing enzymes was discovered. Surprisingly, it was found in a soil sample taken right from his company’s home city of Okayama, Japan. This is how the unique enzymes that produce trehalose were brought into being…but the story goes back a little further.

Proceeding this discovery, research was being conducted all over the world to produce sugar from starch in an efficient way. The major hurdle was that starch was branching half way—like branches of a tree. Researchers knew that if an enzyme that was capable of cutting the branches efficiently could be found, it would be possible to produce diverse sugars with a high degree of purity. During the same time, Hayashibara Co., Ltd. was searching for a microorganism that could produce a branch-cutting enzyme from the mother earth. It was in the spring of 1966, a microorganism that produces this particular enzyme was found under a Japanese persimmon tree in the yard of Hayashibara Co., Ltd.’s research lab in Okayama.

Without the discoveries of these trehalose producing enzymes and branch-cutting enzyme found from the soil in Okayama, it would never have been possible to produce trehalose efficiently.

Future opportunities and perspectives

Trehalose Symposium

Since the launch of trehalose in 1995, Hayashibara has been focusing on applied research to accumulate knowledge on the functional properties and benefits of trehalose. Trehaloses applications continue to expand in the areas of human food, pharmaceuticals and cosmetics products, as well as animal feeds, fertilizers, industrial materials, and preservation of cultural property.
In order to accommodate the year-by-year increase in production volume, the manufacturing plant was extended in 2016, and a system for enhanced production was also established.
In addition, since 1997 Hayashibara has been hosting an annual event called the “Trehalose Symposium”. The symposium provides opportunities for internal and external researchers to present various research results on basic properties of trehalose, its applications for foods, cosmetics, medical care, and agricultural/livestock products.
Hayashibara will continue to pursue the possibilities of trehalose, an inspiring ingredient to taste, health, and beauty.

HB WEB Short version Movie

Hayashibara’s technologies in microorganisms and enzymes, nurtured by accumulated knowledge and experience, create unique functional carbohydrates and dyes for use as active pharmaceutical ingredients or excipients.

High Purity Trehalose

Purity/Other

Preserves higher order
protein structure
TREHALOSE SG

TREHALOSE SG is a low endotoxin product for injection. Stable under heat and acidity, this product stabilizes higher order protein structures during dehydration and freezing by replacing water molecules. TREHALOSE SG prevents cells and proteins from damage.

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Purity/Other
99.0~101.0%/Low Endotoxin
Application
  • Stabilization of antibodies, peptides, and vaccines for injection
  • Additive for culture medium (Ex: Stem cells)
  • Preservation of platelets
Packaging
  • 1kg PE bag
  • 5kg PE bag in a fiber drum
  • 20kg PE bag in a carton box
  • 20kg PE bag in a plastic container
Regulatory Approval
  • JP
  • USP-NF
  • Ph. Eur.
Others
  • US Type IV DMF
  • Halal
Purity/Other

Used as taste improver
TREHALOSE 100PH

Trehalose 100PH can mask unpleasant tastes and odors, improving the quality and acceptability of finished pharmaceuticals such as dry syrups, liquid preparations, and chewable tablets.

Purity/Other
99.0~101.0%
Application
  • Additive for oral solutions (Ex: stabilizer, taste improver, solubility enhancer for minerals)
  • Excipient for solid oral dosage forms (Ex: Tablets)
Packaging
  • 20kg PE bag in a carton box
Regulatory Approval
  • JP
  • USP-NF
  • Ph. Eur.
Others
  • Halal

High Purity Maltose

Purity/Other

Used in infusion fluid
MALTOSE PH

Maltose is a disaccharide containing two glucose molecules. MALTOSE PH has a low endotoxin concentration making it particularly suited for injection and infusion.

Purity/Other
Not less than 98.0%/Low Endotoxin
Application
  • Active ingredient for infusion fluid
  • Solubility enhancer and stabilizer of freeze-dried anti-cancer agents for injection
  • Stabilizer of Immunoglobulins
Packaging
  • 25kg PE bag in a carton box
Regulatory Approval
  • JP
Others
  • US Type II DMF
  • Halal

MALTOSE PH(Milled)

Purity/Other
Not less than 98.0%/Low Endotoxin
Application
  • Solubility enhancer and stabilizer of freeze-dried anti-cancer agents for injection
  • Stabilizer of Immunoglobulins
Packaging
  • 25kg PE bag in a carton box
Regulatory Approval
  • JP
Others
  • Halal

Pullulan

Purity/Other

Acts as a strong binder and
coating agent
PULLULAN

Pullulan has excellent binding strength and film forming properties, which makes it suited for tablets and granules. Low oxygen permeability of PULLULAN maintains potency of active ingredients when used as a coating.

Purity/Other
-
Application
  • Additive for solid oral dosage forms (Ex: Binder, Tablet coating, Granulation)
  • Film
Packaging
  • 10kg PE bag in a carton box
Regulatory Approval
  • JP
  • USP-NF
  • Ph. Eur.
Others
  • Kosher, Halal

PULLULAN(Fine Powder)

Purity/Other
Reference particle size:Not less than 95% pass 250μm
Application
  • Additive for solid oral dosage forms(Ex: Binder, Tablet coating, Granulation)
Packaging
  • 10kg PE bag in a carton box
Regulatory Approval
  • JP
  • USP-NF
  • Ph. Eur.
Others
  • Kosher, Halal

Maltose

Purity/Other

Used in chewable tablets and lozenges
SUNMALT-S

SUNMALT-S is a spray-dried maltose powder that provides excellent flowability, solubility, and compressibility for oral tablet formulations. Maltose is well known for its safety and mouthfeel qualities, especially in chewable tablets and lozenges.

Purity(Maltose)/Other
Not less than 92.0%
Application
  • Excipient for solid oral dosage forms (Ex: Tablets, Granules)
Packaging
  • 20kg Paper bag
Regulatory Approval
  • USP-NF
Others
  • Kosher, Halal

Contact Us

Pharma-Medicals Division,
Life & Healthcare Products Department, NAGASE & CO., LTD.

+81-3-3665-3383(Tokyo)E-Mail

+81-6-6535-2327(Osaka)

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